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. 2016 Mar 22;10:73. doi: 10.3389/fncel.2016.00073

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Copyright © 2016 Bubenheimer, Brown, Fried, McClain and Gulbransen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The percentage of myenteric neurons that express calretinin is not altered by DNBS-colitis in wt or Sirt3^−/− mice. (A–D”) show dual-label fluorescence immunohistochemistry for HuC/D (cyan, A–D) and calretinin (grayscale, A’–D’) in myenteric ganglia from saline- (A–A”, C–C”) or DNBS-treated (B–B”, D–D”) mice. Overlays are shown in (A”–D”). Scale bar in D” = 50 μm and applies to all images. (E) The percentage of HuC/D immunoreactive neurons that also display immunoreactivity for calretinin in healthy and inflamed mice with wt or Sirt3^−/− genotypes (n = 4–5 animals per group, P > 0.05, 2-way ANOVA).