Table 1.
Antibodies used in this study.
| Antibody | Source | Dilution |
|---|---|---|
| Primary antibodies | ||
| Goat anti-Calretinin | Swant, Switzerland; CG1 | 1:1000 |
| Guinea Pig anti-CGRP | Peninsula Labs, San Carlos, CA, USA; T-5053 | 1:500 |
| Chicken anti-GFAP | Abcam, Cambridge, MA, USA; ab4674 | 1:1000 |
| HuC/D Biotinylated | Invitrogen, Carlsbad, CA, USA; A-21272 | 1:200 |
| Sheep anti-nNos | Millipore, Darmstadt, Germany; ab1529 | 1:500 |
| Rabbit anti-Sirt3 | Millipore, Darmstadt, Germany; 07-1596 | 1:100 |
| Rabbit anti-Sirt3 | Cell Signaling, Danvers, MA, USA; 5490 | 1:200 |
| Rabbit anti-Sirt3 | Abcam, Cambridge, MA, USA; Ab86671 | 1:200 |
| Secondary antibodies | ||
| Donkey anti-Rabbit 488 | Jackson, Bay Harbor, ME, USA; 711-545-152 | 1:400 |
| Goat anti-Rabbit 488 | Lifetech, Carlsbad, CA, USA; A-11034 | 1:400 |
| Streptavidin 488 | Jackson, Bay Harbor, ME, USA; 016-540-084 | 1:400 |
| Donkey anti-Goat 568 | Invitrogen, Carlsbad, CA, USA; A-11057 | 1:400 |
| Donkey anti-Sheep 568 | Invitrogen, Carlsbad, CA, USA; A-21099 | 1:400 |
| Goat anti-Chicken 568 | Invitrogen, Carlsbad, CA, USA; A-11041 | 1:400 |
| Donkey anti-Guinea Pig 594 | Jackson, Bay Harbor, ME, USA; 706-585-148 | 1:400 |
| Streptavidin 594 | Jackson, Bay Harbor, ME, USA; 016-580-084 | 1:400 |