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. 2016 Mar 22;7:315. doi: 10.3389/fpls.2016.00315

Figure 5.

Figure 5

PCR analysis of BMYB106 in WT and transgenic lines of birch. (A) PCR of 35S::BpMYB106 transgenic lines. M, DNA Marker DL2000; 1, WT plant; 2, positive control (pROKII-BpMYB106); 3–18, 35S::BpMYB106 transgenic lines. Underlined showed the electrophoresis result of PCR productions. (B) Semi-quantitative RT-PCR analysis of BpMYB106. WT, wild-type plant; lines 1–11; 11 PCR-positive lines. (C) Quantitative RT-PCR analysis of BpMYB106. Genes were normalized using 18S as housekeeping genes. Error bars on each symbol indicate the mean ± SE of three replicate reactions, and statistically significant differences are indicated (*P < 0.05 and **P < 0.01 by two-tailed t-test and one-way ANOVA).