Figure 6.

Expression analysis of 12-oxophytodienoic acid reductase (OPR) gene and OPDA accumulation in response to elicitor treatment. (A) RT-qPCR analysis of OPR at 2, 4, 8, and 24 h after elicitor-treatment. EF1α gene was used as the reference gene. The expression levels in CF-treated plants are relative to the corresponding level of expression in water-treated plants at the indicated time points. Results are reported as means ± standard deviation (SD) of three samples for each treatment. Asterisks indicate a statistically significant difference between the elicitor-treated and the control plants (Student's t-test; ^**P < 0.005). (B) Endogenous OPDA levels in elicitor treated tissues were analyzed at the indicated time points (h). Values are means and standard errors of three independent experiments. Statistical differences were evaluated according to one-way ANOVA followed by Dunnett's test relative to control condition, ^*P < 0.05, and ^**P < 0.01.