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. 2016 Feb 22;35(6):635–653. doi: 10.15252/embj.201592532

Figure EV2. The genome‐wide association of sororin with cohesin occurs exclusively on replicated DNA (related to Fig 4).

Figure EV2

  1. Experimental setup used to synchronize HeLa cells in different states of DNA replication after double thymidine arrest–release (DTAR).
  2. Examples of BrdU sequencing enrichment in early, mid‐, and late S‐phase. Please note the differences in y‐axis scale between different time points. The varying sequencing enrichment possibly represents differences in population‐wide replication dynamics.
  3. Quantification of the co‐occupancy of BrdU incorporation and sororin localization in early, mid‐, and late S‐phase depicted as P‐value distributions and compared to randomized controls. Please note that in late S‐phase, the co‐occupancy between sororin and BrdU is low, presumably because a large part of the genome was replicated and bound by sororin before the BrdU pulse.
  4. Experimental setup to synchronize HeLa cells at two time points corresponding to early S‐phase.
  5. Graph depicting the co‐occupancy of BrdU and sororin at S‐phase time points t1 and t2.
  6. Examples of sororin and SMC3 ChIP‐seq and BrdU DIP‐seq data from early S‐phase as compared to G2‐phase.