Figure 5. DHBP is capable of activating WalKR TCS via targeting erWalK.

(a) Thermal shift assay indicates the direct binding of DHBP to erWalK. (b) DHBP selectively promotes the lysostaphin-induced lysis activity of the Newman wild-type strain, but not the D119A mutant. Cells were grown to OD₆₀₀ of ∼1.4 with the supplementation of 75 μM DHBP (as indicated) before harvesting for analysis. The decline of OD₆₀₀ was utilized to measure lysis activity. The experiments were performed in triplicate. The error bars represent the s.d. of the analysed data.