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. 2016 Mar 18;7:11012. doi: 10.1038/ncomms11012

Figure 5. Switch of miR-122 targeting from TGFβR1 to TGFβ1 generates different metastatic effects in human xenografts or mouse allografts.

Figure 5

(a) Tumour volume was measured after 5 weeks when the indicated cells expressing individual miRNAs or the control hairpin (negative control, NC) cells were subcutaneously implanted into immunodeficient mice. Seven independent repeats are performed in each experiment. (b) Quantitative analysis of the vessel numbers in the indicated tissues. Seven independent repeats are performed in each experiment. (c) Haematoxylin and eosin (H&E) stain of primary tumours 60 days after subcutaneous injection. Mice were subcutaneously implanted with HepG2 cells stably expressing NC, miR-122 or both miR-122 and TGFβ1 (122-TGFβ1), or with Hepa1-6 cells stably expressing NC or miR-122 sponge (122sp) in each group. Seven independent repeats are performed in each experiment. Scale bar, 50 μm. (d) H&E stain of lungs isolated from mice that received tail vein injection of the indicated cells. Seven independent repeats are performed in each experiment. Scale bar, 50 μm. Error bars, ±s.d. *P<0.05; ***P<0.001 by two-sided Student's t-test. ND, no difference.