Figure 2. Impaired production of IFN-γ in the adipose tissue of infected IL-12/IL-23 p40^−/− mice.

Frequencies of (a) IFN-γ⁺ NK1.1⁺ TCRβ⁻TCRγδ⁻ cells on total NK1.1⁺ cells, (b) IFN-γ⁺ NK1.1⁺ TCRβ⁺ TCRγδ⁻ cells on total NK1.1⁺ TCRβ⁺ cells, (c) IFN-γ⁺ TCRγδ⁺ NK1.1⁻ cells on total TCRγδ⁺ cells, (d) IFN-γ⁺ IL-10⁻CD8⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻ cells on total CD8⁺ T cells and (e) IFN-γ⁺ IL-10⁻CD4⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻, IFN-γ⁺ IL-10⁺ CD4⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻ and IL-10⁺ IFN-γ⁻CD4⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻ cells on total CD4⁺ T cells in the mesenteric and subcutaneous adipose tissue (MAT and SAT, respectively) from IL-12/IL-23 p40^−/− mice sacrificed 24 h after intraperitoneal challenge with 1 × 10⁷ N. caninum tachyzoites (NcT) or PBS, as indicated. Each symbol represents an individual mouse. Bars represent means of 7–9 mice per group pooled from 2 independent experiments. (Mann-Whitney U, **P ≤ 0.01). Representative example of gating strategy used to define the respective cellular populations in the different depots of adipose tissue analysed. The example shown corresponds to MAT.