Figure 5.

Sharp increase in the frequency of TCRγδ⁺ IFN-γ⁺ cells in the adipose tissue of N. caninum infected mice. Frequencies of (a) IFN-γ⁺ NK1.1⁺ TCRβ⁻TCRγδ⁻ cells on total NK1.1⁺ cells, (b) IFN-γ⁺ NK1.1⁺ TCRβ⁺ TCRγδ⁻ cells on total NK1.1⁺ TCRβ⁺ cells, (c) IFN-γ⁺ TCRγδ⁺ NK1.1⁻ cells on total TCRγδ⁺ cells, (d) IFN-γ⁺ CD8⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻ cells on total CD8⁺ T cells and (e) IFN-γ⁺IL-10⁻CD4⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻, IFN-γ⁺ IL-10⁺ CD4⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻ and IL-10⁺ IFN-γ⁻CD4⁺ TCRβ⁺ TCRγδ⁻NK1.1⁻ cells on total CD4⁺ T cells in the gonadal, mesenteric, omental and subcutaneous adipose tissue (GAT, MAT, OAT and SAT, respectively) and mesenteric lymph nodes (MLN) observed 7 days after intraperitoneal challenge with 1 × 10⁷ N. caninum tachyzoites (NcT) or PBS, as indicated. Each symbol represents an individual mouse. Bars represent means of 9 mice per group pooled from 3 independent experiments. (Mann-Whitney U, *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001). Representative example of gating strategy used to define the respective cellular populations in the different depots of adipose tissue analysed. The example shown corresponds to MAT.