Figure 2.

Siglec-G Deficiency Decreases Atherosclerosis in Cholesterol-Fed Ldlr^−/−Siglec-G^−/− Mice

(A) Quantitative analysis of atherosclerosis in the aorta. The data are expressed as percentage of Sudan IV stained area of the entire aorta. The representative pictures are shown on the right.

(B) Quantitative analysis of atherosclerotic lesions in cross-sections of innominate arteries. The values represent the average μm² of four sections throughout the innominate artery (300 μm). The images show representative Masson’s trichrome stains. The original magnification is 50×, and the scale bar represents 200 μm.

(C) Quantification of atherosclerotic lesion size in cross-sections at the aortic origin. The values represent the average μm² of nine sections throughout the entire aortic origin (400 μm). The images show representative Masson’s trichrome stains. The original magnification is 50Χ, and the scale bar represents 200 μm.

(D) Quantification of necrotic areas in cross-sections at the aortic origin. The values represent the percentages of necrotic areas of one section at 200 μm depth.

(E–G) Quantification of macrophage, collagen, and IgM content in cross-sections at the aortic origin.

(E) Values represent the percentage of mac-3⁺ area per cellular lesion area of one section per mouse at 200 μm depth.

(F) Values represent the percentage of Sirius Red⁺ area per total lesion area of one section per mouse at 200 μm depth.

(G) Values represent the percentage of IgM⁺ area per cellular lesion area of one section per mouse at 180 μm depth. Shown are the data of Ldlr^−/− and Ldlr^−/−Siglec-G^−/− mice after 8 weeks of atherogenic diet. The symbols represent individual mice. The horizontal bars represent the mean of each group and errors bars represent SEM (^∗p ≤ 0.05, ^∗∗p ≤ 0.01, and ^∗∗∗p ≤ 0.001).

See also Table 1.