Figure 5.
Epithelial-Mesenchymal Plasticity Renders Potential Stem Cell Differences Irrelevant for Metastatic Outgrowth
(A) The percentage of E-cadLO- and E-cadHI-circulating tumor cells. n = 13 mice.
(B) Representative images of single E-cadLO and E-cadHI cells and a multi-cellular metastasis in the lung. White rectangle highlights single cells. Scale bars, 20 μm.
(C) Percentages of E-cadLO and E-cadHI tumor cells in blood and lungs. Blood: n = 13 mice; lungs: n = 143 metastases in 16 mice.
(D) Representative images of liver metastases grown from E-cadHI cells (left) and E-cadLO cells (right). Scale bars, 40 μm.
(E) Table indicating the metastatic outgrowth potential of E-cadLO and E-cadHI cells. Tumor-initiating cell frequency as tested by the Elda-limiting dilution test: E-cadHI cells 1/21,228; E-cadLO cells 1/17,545, p = 0.82.
Related to Figure S5.