Figure 2. FGF2-binding property of the selected hits, analyzed with an indirect assay, as ability to compete with the TSP-1 fragment E123CaG1 (comprising the type III repeats domain) for FGF2 binding, as described in Methods.

Binding of the biotinylated recombinant domain to FGF2 in the presence or absence of each molecule (5 μM). Data are the percentage of control binding, mean and SE of 3 experiments performed in triplicate. *p < 0.05 compared to control (ANOVA followed by Bonferroni test).