Table 1. FGF2 binding ability of the selected hits (competition assay).
| Group | Molecule | IC50 (μM) |
|---|---|---|
| Initial query | SM27 | 3.90 ± 0.23 |
| Bi-naphthalene | SM.2–16 | 0.59 ± 0.09 |
| Bi-naphthalene | SM.2–18 | 0.61 ± 0.06 |
| Bi-naphthalene | SM.2–19 | 0.35 ± 0.03 |
| Bi-naphthalene | SM.2–20 | 0.50 ± 0.11 |
| Bi-naphthalene | SM.2–22 | 0.42 ± 0.09 |
| Bi-naphthalene | SM.2–23 | 0.53 ± 0.11 |
| Bi-naphthalene | SM.2–24 | 0.46 ± 0.10 |
The ability of the selected hits to compete for the binding of the recombinant TSP-1 domain to FGF2 was analyzed as an indirect measure of their ability to bind FGF2, as previously described15. Data are the IC50 (in μM), mean ± SE of data from 3 experiments.