Figure 1. Validation of BRE null mutation in fibroblasts isolated from BRE knockout mice.

(a) PCR-based genotyping of WT and BRE^−/− fibroblasts showing homozygous deletion of BRE exon 3 specifically in the latter. (b) BRE mRNA expression in WT and BRE^−/− fibroblasts as determined by RT-qPCR. House-keeping gene GAPDH was used for normalization. (c) BRE protein expression in WT and BRE^−/− fibroblasts by Western blotting with α-tubulin as protein loading control. (d) Immunofluorescence staining of WT and BRE^−/− fibroblasts using anti-BRE antibody. Nuclei were counterstained with DAPI. BRE was expressed in both the cytoplasmic and nuclear compartments of WT fibroblasts, but none in the BRE^−/− fibroblasts.