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. 2016 Mar 22;6:23506. doi: 10.1038/srep23506

Figure 7. The recruitment of BRCA1-A complex and HR-dependent DNA repair are impaired in BRE−/− fibroblasts.

Figure 7

(a) mRNA expression of BRCA1-A complex components in WT and BRE−/− fibroblasts as determined by RT-House-keeping gene GAPDH was used for normalization. Note the significant downregulation of BRCA1, BRCA2 and BARD1 in BRE−/− fibroblasts. Data represent the mean ± SD of triplicate wells. **denotes P-value < 0.01, ***P-value < 0.001 versus WT. (b) The foci formation of BRCA1 and RAP80 at 24, 48 and 72 h after 10 Gy of gamma irradiation as examined by immunofluorescence staining, together with DAPI nuclear staining. (c) RAD51 and γ-H2AX foci formation by immunofluorescence staining at 2, 8 and 24 h after 10 Gy of gamma irradiation. Scale bars = 10 μm. Note the weaker BRCA1, RAP80 and RAD51 foci staining of BRE−/− fibroblasts compared with the WT.