Table 1.
Quantification of Irisin in Plasma from Human Subjects Skyline software was used to quantify absolute amounts of irisin peptides from the plasma of sedentary and aerobically trained subjects. The 25 kDa glycosylated bioactive form of irisin was used to calculate its ng/ml concentrations in plasma.
| Sedentary | N-terminal peptide (femtomoles) | Internal (femtomoles) | combined average (femtomoles) | ng/mL |
|---|---|---|---|---|
| Subject 1 | 16.66 | 12.78 | 14.72 | 3.68 |
| Subject 2 | 11.98 | 12.3 | 12.14 | 3.03 |
| Subject 3 | 13.73 | 15.59 | 14.66 | 3.66 |
| Subject 4 | 16.21 | 16.7 | 16.455 | 4.11 |
| Average | 14.65 | 14.34 | 14.49 | 3.62 |
| Aerobic | N-terminal peptide (femtomoles) | Internal (femtomoles) | combined average (femtomoles) | ng/mL |
|---|---|---|---|---|
| Subject 1 | 13.98 | 14.78 | 14.38 | 3.66 |
| Subject 2 | 18.22 | 19.74 | 18.98 | 4.74 |
| Subject 3 | 16.11 | 22.22 | 19.165 | 4.79 |
| Subject 4 | 16.28 | 17.38 | 16.83 | 4.28 |
| Subject 5 | 16.68 | 19.2 | 17.94 | 4.48 |
| Subject 6 | 15.1 | 17.11 | 16.105 | 4.02 |
| Average | 16.06 | 18.41 | 17.23 | 4.33 |