Table 1.
Manufacturing systems for the production of gene therapy viral vectors organized by system, vector and method
| System | Vector | Method and cells | References |
|---|---|---|---|
| HYPERFlask (Corning) | LV vector | Transfection, HEK 293T adherent cells (CaPhos) | (48) |
| Roller Bottles | AAV | Transfection HEK293 adherent cells (CaPhos) | (49,50) |
| γ-Retroviral vector | PG13 and GPE-Am12-based stable producer cell lines | (51) | |
| Cell Factories (Nunc) or CellSTACKS (Corning) | LV vector | Transfection, HEK293 or 293T adherent cells (CaPhos, Lipofectamine 2000) | (52–55) |
| γ-Retroviral vector | PG13-based stable producer cell line; Transfection, HEK 293T adherent cells (CaPhos) | (35,56) | |
| AAV | Transfection, HEK293 (CaPhos) | (57) | |
| Foamy virus | Transfection, 293T, (PEI) | (58,59) | |
| iCELLis™ (Pall) | Adenoviral vector | Infection | (60) |
| AAV | Transfection, HEK 293T (PEI) | (61) | |
| γ-Retroviral vector | PG13 and 293Vec stable producer cell lines | (62) | |
| LV vector | Transfection, 293T adherent cells (CaPhos) | (63) | |
| Spinner Flask, Stirred Bioreactor | γ-Retroviral vector | Suspension-based stable producer cell lines, PA317-based stable producer cell line on Cytodex 1, 2 and Cytopore 2 microcarriers | (64,65) |
| Adenovirus | PA317-based stable producer cell line on Cytodex 1, 2 and Cytopore 2 microcarriers | (64) | |
| Rabies virus | Vero cells on Cytodex 1 microcarriers | (66) | |
| Baculovirus/AAV | SF9 cells in suspension infected using baculovirus | (67–72) | |
| LV vector | Transfection, HEK293, HEK293SF-3F6, suspension (PEI) | (73,74) | |
| CellCube (Costar) | γ-Retroviral vector | 203T Phoenix amphotropic packaging cell lines and TE Fly GA18 stable producer cell line | (75,76) |
| HSV-1 | Vero cell-derived 7b complementing cell line | (77) | |
| Wave Bioreactor (GE Healthcare) | γ-Retroviral vector | Transfection, HEK 293T adherent cells on FibraCel (CaPhos) | (35,36) |
| LV vector | GPRG stable producer cell line on FibraCel; producer cell lines suspension | (78,79) | |
| AAV | Transfection, HEK293, suspension (PEI); HeLa-based producer cell line/Ad5 infection; BHK-21 cells/HSV-1 infection | (80–83) | |
| Chemap CF-2000 bioreactor (Mannedorf) | LV vector | Transfection, 293E suspension cells (PEI) | (84) |
| AAV | Transfection, HEK293, 293E, 293F suspension cells (PEI) | (85) | |
| Spinferm Bioreactor (Braun Biotechnology) | AAV | Transfection, HEK293 suspension cells (CaPhos) | (86) |
| Celligen Plus (NewBrunswick Scientific) | Adenovirus | Infection, HEK293 | (7) |