Fig. 1.

CHRFAM7A and CHRNA7 gene expression in inflammatory bowel disease (IBD). Quantitative RT-PCR was used to measure CHRFAM7A and CHRNA7 gene expression in intestinal biopsies from patients with IBD (panels A–C), ulcerative colitis (panels D–F), and Crohn's disease (panels G–J). Gene expression in each sample was normalized to that of actin or, as indicated, between each other to determine the CHRFAM7A/CHRNA7 ratio. Relative gene expression was compared to that in control biopsies using the ∆∆Ct method. Differences in gene expression between the control and disease biopsies were measured by REST [30] for group-wise comparisons and evaluated as either individual plates and after combination (shown).