Fig. 2.

Quantified oncogenes in GB06. An example of multiplex ligation-dependent probe amplification (MLPA) results for 78 oncogenes in one glioblastoma. Its high level amplifications of PDGFRA and EGFR were readily detected as fluorescence signals from each of the 2 sets of probes used for each oncogene. The two-fold elevations in tumor versus normal brain were detected using mathematical comparisons described in Section 2.2. The red and blue lines represent tumor and normal DNA, respectively. Also, the inset at the top shows fluorescence in situ hybridization (FISH) signals for EGFR (red) in two cells of the frozen tissue sample used for MLPA. The nuclei stained blue with diaminophylindole (DAPI).