Fig. 4.

Expressions of MTB/GF genes and the effect of ENO1 transformation on ACLY in considering ENO1 as a candidate for MTB/GF transformations. (a) Comparisons of MTB/GF gene expressions, including ENO1, prior to transformations in 22 glioblastomas (GBs). Normal brain RNA was from pooled non-malignant brain tissue. Each gene's fold change was obtained with the ddCt method described in Section 2.3. Means with 95% confidence intervals are shown for each gene. (b) Effects of ENO1 when used to transform a metabolic gene's expression data. On the left, the distribution of GBs, each identified with a 2-digit number, is displayed according to ACLY's expression in each as a fold-change compared to normal brain's ACLY RNA. The distribution shown on the right is according to each tumor's expression of ACLY following transformation (division by concurrent ENO1 expression).