LC3 and p62 modulation after EBSS±Baf. A1 treatment in four cell models. Cells were treated with EBSS, Baf. A1 and dual treatment for 4 h, as described in Section 2. Representative blots of 3 independent experiments of MEFs (A), HFs (D) SH-SY5Y (G) and N27 rat dopaminergic cells (J) are shown. GAPDH was used as a loading control. p62 densitometry expressed in arbitrary units is shown in panels B (MEFs), E (HFs), H (SH-SY5Y) and K (N27). LC3 (isoforms I and II) densitometry expressed in arbitrary units is shown in panels C (MEFs), F (HFs), I (SH-SY5Y) and L (N27). Data represent the mean±SEM; n=3 (*p≤0.05, **p≤0.01, ***p≤0.001, related to the corresponding non-treated condition).