p62 mRNA analysis after EBSS±Baf. A1 treatment in four cell models. Cells were treated with EBSS, Baf. A1 and dual treatment for 4 h, as described below. Quantification of p62 mRNA expression levels from all lines studied is shown: MEFs (A), HFs (B), SH-SY5Y (C) and N27 cells (D). Relative expression was determined using GAPDH as a housekeeping gene. Data represent the mean±SEM; n=3 (**p≤0.01, related to the corresponding non-treated condition).