Subject area |
Biochemistry and Molecular Biology |
More specific subject area |
Autophagy |
Type of data |
Figures |
How data was acquired |
Western-blotting (SDS-gel electrophoresis and semi-dry transfer; Bio-Rad equipment and ImageJ software), immunofluorescence (inverted microscope (OLYMPUS IX-51) and ImageJ software), quantitative PCR (qPCR) (Applied Biosystems 7500 PCR real system), data statistical analysis (SPSS software). |
Data format |
Analyzed |
Experimental factors |
Four cell lines (Mouse embryonic fibroblasts (MEFs), Human fibroblasts (HFs), SH-SY5Y human neuroblastoma cells, and N27 rat dopaminergic cells). Starvation-induced autophagy by incubation with Earle׳s Balanced Salt Solution (EBSS) medium, bafilomycin A1 (Baf. A1) treatment (blocking autophagosome-lysosome fusion), and dual treatment (Baf. A1+EBSS). |
Experimental features |
Western-blotting analysis of LC3 and p62 proteins using Sample buffer (SB) 1X lysis buffer, detection of endogenous LC3 and p62 immunofluorescence and p62 mRNA levels by qPCR. |
Data source location |
Not applicable |
Data accessibility |
Data is within this article |