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. 2016 Mar 9;7:641–647. doi: 10.1016/j.dib.2016.02.085
Subject area Biochemistry and Molecular Biology
More specific subject area Autophagy
Type of data Figures
How data was acquired Western-blotting (SDS-gel electrophoresis and semi-dry transfer; Bio-Rad equipment and ImageJ software), immunofluorescence (inverted microscope (OLYMPUS IX-51) and ImageJ software), quantitative PCR (qPCR) (Applied Biosystems 7500 PCR real system), data statistical analysis (SPSS software).
Data format Analyzed
Experimental factors Four cell lines (Mouse embryonic fibroblasts (MEFs), Human fibroblasts (HFs), SH-SY5Y human neuroblastoma cells, and N27 rat dopaminergic cells). Starvation-induced autophagy by incubation with Earle׳s Balanced Salt Solution (EBSS) medium, bafilomycin A1 (Baf. A1) treatment (blocking autophagosome-lysosome fusion), and dual treatment (Baf. A1+EBSS).
Experimental features Western-blotting analysis of LC3 and p62 proteins using Sample buffer (SB) 1X lysis buffer, detection of endogenous LC3 and p62 immunofluorescence and p62 mRNA levels by qPCR.
Data source location Not applicable
Data accessibility Data is within this article