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. 2016 Feb 17;5(2):e002701. doi: 10.1161/JAHA.115.002701

Figure 10.

Figure 10

Detection of oxidative stress in the brains of wild‐type and heterozygous SHR ‐Ndufc2 em1Mcwi at the end of either RD or JD exposure. A, Detection of carbonylated proteins using the Oxyblot Protein Oxidation Detection kit (n=3; Millipore, Milan, Italy) in brains of heterozygous SHR‐Ndufc2 em1Mcwi versus parental lines at the end of 4 weeks of exposure to either RD or JD. B, Corresponding densitometric analysis with values shown as means±SD. *P<0.0001 for SHRSP JD versus SHRSP RD and for both versus all other samples. Densitometric values are expressed as means±SD. C, Detection of carbonylated proteins using the Oxyblot Protein Oxidation Detection kit (n=3) in brains of wild‐type SHR‐Ndufc2 em1Mcwi versus parental lines at the end of 4 weeks of exposure to either RD or JD. D, Corresponding densitometric analysis with values shown as means±SD. *P<0.0001 for SHRSP JD versus SHRSP RD and for both versus all other samples. Het indicates heterozygous; JD, Japanese‐style stroke‐permissive diet; RD, geular diet; SHRSP, stroke‐prone spontaneously hypertensive rat; SHRSR, stroke‐resistant SHR; wt, wild type.