Figure 6.

Accumulation of CD4⁺ cells but not CD8⁺ cells or CD68⁺ cells after allogeneic MSC sheet placement. Representative images of immunohistostaining for accumulation of CD4⁺, CD8⁺, and CD68⁺ cells in the MSC sheets at day 3 after treatment (Figure 5A through 5C, respectively) are shown (A through C). Right panels are higher magnification images of white rectangle areas in the left panels. Only positive cells with nuclei (white arrows) were counted for comparison. Scale bars=50 μm. MSC indicates mesenchymal stromal cell.