Figure 7.

Allogeneic MSC sheets evoked a low‐level immunologic response at early phase after epicardial placement. A, Quantitative RT‐PCR detected that IL6, but not many other key genes involved in immunologic responses, was upregulated in the left ventricular myocardium (including the MSC sheets) at day 3 in the allo group compared with the auto groups. B, Enzyme‐linked immunosorbent assay demonstrated that serum levels of IL‐6 were similarly low (detection limit) at day 3 after treatment in all groups. C, Quantitative RT‐PCR showed that myocardial upregulation of molecules relevant to immunologic responses observed at day 3 in the allo group (A) was diminished by day 28 after treatment. *P<0.05 vs sham. ^† P<0.05 vs auto. n=5 per group (A through C). Allo indicates allogeneic MSC sheet placement at 4 weeks after myocardial infarction; Auto, syngeneic MSC sheet placement at 4 weeks after myocardial infarction; MSC, mesenchymal stromal cell; RT‐PCR, reverse transcription polymerase chain reaction; Sham, sham treatment (open chest only) at 4 weeks after myocardial infarction.