Subject area	Biology
More specific subject area	Mass spectrometry-based identification of allergen
Type of data	Figure, Table
How data was acquired	MALDI-TOF/TOF (Autoflex II, Bruker Daltonics, Germany) and LC-ESI qTOF (maXis Impact, Bruker Daltonics, Germany) analysis of in-gel digested proteins
Data format	Analyzed mass spectrometry data
Experimental factors	Total pollen proteome of Helianthus annuus was confronted with pooled sera of sunflower pollen-sensitized patients. Allergenic proteins were excised from 2D gel, and in-gel digested spots were identified using a combination of two different mass spectrometric techniques (MALDI TOF/TOF and LC-ESI qTOF) and searching the MS/MS data against NCBInr database and EST library of H. annuus.
Experimental features	Trypsin digested IgE reactive proteins were subjected to MALDI-TOF/TOF. The raw MS/MS spectra were initially searched against NCBInr database. Unmatched peptides with low significance score were further subjected to similarity searches against the H. annuus EST database Unmatched proteins, after MALDI-TOF/TOF, were subjected to LC-ESI qTOF for better peak resolution, maximum signal, and maximum coverage. They were loaded on nano-LC-ESI MS/MS. Similarly, MS/MS spectra were searched against NCBInr and EST library of H. annuus. Finally, FASTA sequences of matched EST clones were analyzed by BLASTx algorithm.
Data source location	Bose Institute, Kolkata, India
Data accessibility	Data available within this article and accessible at ProteomeXchange Consortium via PRIDE partner repository with the dataset identifier PRIDE: PXD002397.