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. 2016 Mar 22;7:45. doi: 10.1186/s13287-016-0306-3

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© Webb et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — RAR agonists can prevent tenogenic differentiation and other instructed mesenchymal lineages in rat ATSCs (passage 6) following long-term differentiation in confluent conditions a–i. Stimulation with osteogenic d, g, adipogenic e, h, and chondrogenic f, i media caused a time-dependent increase in alkaline phosphatase d, g, LipidTOX-Green/Foxo1 e, h and Aggrecan/Col2a1 f, i respectively. Addition of tazarotene to the induction media causes a decrease in all differentiation markers a–i. Values are given as the mean ± SD, n = 3. *p <0.05, **p <0.005, ***p <0.001. DMSO dimethyl sulfoxide