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. 2016 Mar 22;47:47. doi: 10.1186/s13567-016-0332-2

Figure 3.

Figure 3

Effect of BHV-1 on mitochondrial membrane potential in MDBK cells. (A) MDBK cells of confluent in 96-well plates were infected with BHV-1 at MOI of 0.01 for indicated time length, and then MMP was detected with JC-1 dye. The treatment of cells with CCCP for 20 min was used as a positive control. (B) MDBK cells of confluent in 96-well plates were treated with NAC and GSH at indicated concentrations, before and during virus infection with BHV-1 (MOI = 0.01) for 48 h. The MMP was detected with JC-1 dye. Data represent three independent experiments. Statistical analyses were performed using Student’s t test (Asterisk indicates P < 0.05 vs. mock).