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. 2016 Mar 22;47:47. doi: 10.1186/s13567-016-0332-2

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© Zhu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of BHV-1 on ATP content during the infection of MDBK cells. MDBK cells of confluent in 6-well plates were infected with BHV-1 at MOI of 0.01 for indicated time length. The content of ATP and protein concentration was determined with ATP Assay Kit and Bradford Protein Assay Kit, respectively. The ATP content was normalized to the protein concentrations and expressed as a % of mock treated control cells. Data represent three independent experiments. Statistical analyses were performed using Student’s t test (Asterisk indicates P < 0.05 vs. control).