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. 2016 Mar 22;13:51. doi: 10.1186/s12985-016-0505-7

Table 1.

The recombinant viruses used in this study and the related mutant amino acid sites

Name aa mutant sites Name aa mutant sites
rBB No rBB20s No
rBB/GP2s GP2(198G/V) rBB20s/GP2 GP2(198 V/G)
rBB/GP3s GP3(69P/S,71 K/R,226S/P) rBB20s/GP3 GP3(69S/P,71R/K,226P/S)
rBB/GP4s GP4(43D/N;44 F/S) rBB20s/GP4 GP4(43 N/D;44S/F)
rBB/Ms M(70R/K) rBB20s/M M(70 K/R)
rBB/Ms-R a No rBB20s/M-R b No

a The recombinant virus rBB/Ms-R was a revertant virus from rBB/Ms and contained 70R in M. The plasmid was constructed from the full-length infectious cDNA clone pCMV-BB/Ms by using the site-directed mutagenesis described

b The recombinant virus rBB20s/M-R was a revertant virus from rBB20s/M and contained 70 K in M. The plasmid was constructed from the full-length infectious cDNA clone pCMV-BB20s/M by using the site-directed mutagenesis described