Abstract
Rapid methods depending on differential absorptiometry are described for the determination of the transferrin iron content and the latent iron-binding capacity of blood serum. Each determination requires as little as 0·5 ml. serum. The methods are well adapted for routine use in the `average' laboratory. Three or four sera may be completely analysed in 30 minutes. All operations are carried out in the cells or tubes used for the colorimetric measurements, no precipitation or heating being employed at any stage. Critical investigations of the reliability of the methods are attempted and ranges of normal values are included.
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Selected References
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- Cartwright G. E., Wintrobe M. M. CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XXXIX. THE ANEMIA OF INFECTION. STUDIES ON THE IRON-BINDING CAPACITY OF SERUM. J Clin Invest. 1949 Jan;28(1):86–98. doi: 10.1172/JCI102058. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIES G., LEVIN B., OBERHOLZER V. G. The micro estimation of serum iron and iron-binding capacity in normals and in disease. J Clin Pathol. 1952 Nov;5(4):312–316. doi: 10.1136/jcp.5.4.312. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENRY R. J., SEGALOVE M. The running of standards in clinical chemistry and the use of the control chart. J Clin Pathol. 1952 Nov;5(4):305–311. doi: 10.1136/jcp.5.4.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KALDOR I. A shorter method for determining the latent iron-binding capacity of serum. Med J Aust. 1955 Nov 26;42(22):886–889. [PubMed] [Google Scholar]
- LAURELL C. B. Plasma iron and the transport of iron in the organism. Pharmacol Rev. 1952 Dec;4(4):371–395. [PubMed] [Google Scholar]
- Rath C. E., Finch C. A. CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XXXVIII. SERUM IRON TRANSPORT. MEASUREMENT OF IRON-BINDING CAPACITY OF SERUM IN MAN. J Clin Invest. 1949 Jan;28(1):79–85. doi: 10.1172/JCI102057. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TRINDER P. The improved determination of iron in serum. J Clin Pathol. 1956 May;9(2):170–172. doi: 10.1136/jcp.9.2.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VENTURA S. Determination of the unsaturated iron-binding capacity of serum. J Clin Pathol. 1952 Aug;5(3):271–274. doi: 10.1136/jcp.5.3.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEBSTER D. The determination of serum iron after the intravenous injection of iron-dextran. J Clin Pathol. 1960 May;13:246–248. doi: 10.1136/jcp.13.3.246. [DOI] [PMC free article] [PubMed] [Google Scholar]