Fig. 3.

A direct target gene of p53, Nedd4-2, mediates neural network synchrony. a Representative western blots of Nedd4-2 and Gapdh from cortical lysates of WT or Nedd4-2 ^andi mice. b Representative traces of recordings from 2 electrodes that are over 400 μm away from each other. Selected electrodes are marked with “1” and “2” on the electrode map (left). Traces from the electrodes of WT or Nedd4-2 ^andi cultures before or after 24-h treatments are shown on the right. c ₁ Quantification of synchrony index from entire MEA of WT or Nedd4-2 ^andi cultures before and after PTX treatments. c ₂ Quantification of synchrony index from entire MEA of Nedd4-2 ^andi cultures before and after Pifithrin-α + PTX treatment. d Representative traces of neural network bursts from WT or Nedd4-2 ^andi cultures treated as indicated. Traces are from a designated electrode before and after treatment. e ₁ Quantification of the number of spikes per burst from WT or Nedd4-2 ^andi cultures before and after PTX treatments. e ₂ Quantification of the number of spikes per burst from Nedd4-2 ^andi cultures before and after Pifithrin-α + PTX treatment. Plotted is the average data normalized to vehicle control from WT cultures before PTX treatment. ANOVA with post-hoc Tukey test was used. (n = 4–7 cultures, *p < 0.05, ***p < 0.001, ns: non-significant)