Fig. 4.

Mdm2-p53-Nedd4-2 signaling regulates seizure susceptibility. a Western blots of Mdm2, pMdm2-(S166), p53, Nedd4-2, and Gapdh from cortical lysate of 6–8 weeks old WT C57BL/6 male mice 1 h after kainic acid injection (60 mg/kg). (n = 4 littermates per condition, One-sample t-test, *p < 0.05; **p < 0.01). b 6–8 weeks old WT (BALB/cByJ background) and c Nedd4-2 ^andi mice (also in BALB/cByJ background) were intraperitoneally injected with (right) or without (left) a single dose of Pifithrin-α (2 mg/kg) for 2 days prior to kainic acid injection (15, 30 or 60 mg/kg). The number of mice used in each condition is shown on the top of each bar. Mice showing stage 4 and/or 5 seizure are counted as 1 while mice showing stage 3 seizure or under are counted as 0. The statistical results after ANOVA with post-hoc Tukey test are as below: WT vs WT + Pifithrin-α, *p < 0.05; WT vs Nedd4-2 ^andi, *p < 0.05; Nedd4-2 ^andi vs Nedd4-2 ^andi + Pifithrin-α, p > 0.05