Fig. 5.

Manipulation of ITGA6 levels directly impacts invasion. a Non-sorted, cultured shControl or shHIF1A/shHIF2A MDA-MB-231 cells were evaluated for invasive potential using Boyden chamber assays. The grand mean of the invasion index (invasion corrected for random migration) ± SEM of 3 independent experiments is shown; p-values calculated by Student’s t-test are indicated. b Knockdown of ITGA6 impairs invasion at normoxia and hypoxia. The grand mean of the invasion index (invasion corrected for random migration) ± SEM of 3 independent experiments is shown; p-values were calculated by Student’s t-test. c Ectopic expression of ITGA6 in shHIF1A/shHIF2A cells partially rescues invasion at hypoxia. The grand mean of the invasion index (invasion corrected for random migration) ± SEM of 3 independent experiments is shown; p-values were calculated by Student’s t-test.