Table 1.

Binding Parameters of 1–3 to PrfA_WT and PrfA_G145S as Determined by ITC

Protein	Compound	KD (M)	ΔHA (kcal/mol)	ΔSA (cal/mol·K)
PrfAWT	1	1.0 (±0.2) × 10−6	−8.4 ± 0.1	−0.8 ± 0.1
2	6.7 × 10−6	−6.5	2.8
3	1.6 (±0.2) × 10−6	−8.4 ± 0.2	−1.5 ± 0.9
PrfAG145S	1	7.75 × 10−6	−6.2	2.6
2	5.4 × 10−6	−4.1	10.4
3	5.0 (±1.8) × 10−6	−6.2 ± 1.0	3.4 ± 4.1

ΔH_A, enthalpy of association and ΔS_A, entropy of association. Determined at 293 K. In all cases, 1 to 1 stoichiometry per PrfA monomer was observed. Errors refer to independent repetitions (2–3) of experiments. Fitting errors not shown. See also Figures 5 and S3.