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. 2015 Nov 17;10(1):55–68. doi: 10.1080/19336950.2015.1108499

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Figure 2. — CT_1505-1671 (IVT) interaction with GST-NT_1-154 in the presence of Ca²⁺ or EGTA, with or without CaM. (A) Pull down experiment of radioactively labeled pCTs, pulled by GST-NT_1-154. Radiograms of the labeled proteins are shown. (B) Average of 4-6 pull down experiments as in A. Each group of experiments is normalized to the conditions of EGTA with no CaM of the same group. 0.2 mM was calculated with the winmaxc32 software. ** p < 0.01. All the experiments were done with 3 µg GST-NT_1-154 and CaM.