Figure 5.
Effect of NT mutations on ICa and IBa. (A) 129-154 NT a.a. arranged in triples or quadruples which were mutated to alanines. (B) Inactivation of the channel with Ba2+ and Ca2+. From left to right: 1st column, normalized averaged IBa (black) and ICa (red) currents (3-15 oocytes in each group) measured at +20 mV. 2nd column, voltage-conductance (G-V) curve. 3rd column, fraction of peak current after 400 ms depolarization (r400) of IBa and ICa at different voltages. 4th column, fraction of peak current after 400 ms depolarization (r400) of IBa and ICa at 20 mV, *P < 0.05. 5th column, the difference between r400 of IBa and ICa (f400), *P < 0.05.