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. 2015 Sep 23;6(5):79–90. doi: 10.1080/21541264.2015.1095269

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — CTCF controls P-TEFb recruitment/activity and recognition of the U2 3’ box. (A,B) ChIP analysis of the CDK9-subunit of P-TEFb, phosphorylation of Ser2 (pSer2) of the CTD and pSer2 related to RNAP II on c-myc (A) and U2 (B) in control and CTCF K_D cells. (C) RNase protection analysis of transcripts from U2 in control and CTCF K_D cells. The U2 pre-snRNA (pre-U2), the readthrough (U2 RT) and the readaround (U2 RA) are indicated. The ratio of RT/preU2 and RA/preU2 results from the quantification of 3 independent experiments and associated standard deviation is indicated.