Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 13;6(5):91–101. doi: 10.1080/21541264.2015.1114983

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 3. — Cytoplasmic - nuclear distribution of Kme_1/2 methylated RNAPIIA. (A) Cytoplasmic and nuclear extracts of H1299 cells were prepared and analyzed in Western blots. The ratio of RNAPIIA to RNAPII0 in the cytoplasm and nucleus was visualized with mAbs Pol3.3 and Ser2P. H3K4me3 served as nuclear markers and tubulin as cytoplasmic marker. (B) Immunofluorescence staining of formaldehyde fixed H1299 cells. Ser2P, Ser5P, and Ser7P modified CTD was observed exclusively in the nucleus, while significant CTD Kme_1/2 staining was observed also for the cytoplasm. Merge: overlay of DAPI and Cy3; PhC: phase contrast.