Table 1.
Effect of additives on cellulose conversiona and apparent inhibition degrees
| Additives | Cellulose conversion without vanillin (%) | Cellulose conversion with vanillin (%) | Apparent inhibition (%) |
|---|---|---|---|
| Blankb | 41.9 ± 0.5 | 26.8 ± 0.6 | 35.9 ± 0.6 |
| CaClb2 | 42.8 ± 0.2 | 27.0 ± 1.3 | 36.7 ± 0.5 |
| Tween 80b | 42.0 ± 1.5 | 28.5 ± 1.5 | 32.2 ± 1.5 |
| Granular activated carbonb | 39.7 ± 0.8 | 24.8 ± 1.4 | 37.5 ± 1.1 |
| Powdered activated carbonb | 28.6 ± 0.6 | 14.1 ± 1.7 | 50.5 ± 1.0 |
| BSAb | 42.0 ± 1.0 | 27.5 ± 0.3 | 34.6 ± 0.6 |
| Blankc | 41.9 ± 0.5 | 32.4 ± 1.0 | 22.6 ± 0.3 |
| BSAc | 40.8 ± 0.3 | 31.5 ± 0.3 | 22.8 ± 0.3 |
| BSAc, d | 40.8 ± 0.8 | 31.5 ± 1.0 | 23.2 ± 0.9 |
aEnzymatic hydrolysis of Avicel was carried out with cellulose concentration of 10 mg/mL and cellulase concentration of 0.3 mg/mL at 50 ºC. Cellulose conversion was determined at 24 h. The concentration of the additives was 2.5 mg/mL
bVanillin concentration was 5 mg/mL
cVanillin concentration was 2.5 mg/mL
dVanillin was pre-incubated 3 h with BSA before enzyme addition