Table 2.

Effect of pH on vanillin inhibition

PH	Cellulose conversiona without vanillin (%)	Cellulose conversion with vanillinb (%)	Apparent inhibition (%)	Inhibitor-binding constant β (vanillin, mL/mg)
4.3	40.9 ± 2.1	26.3 ± 1.8	35.7 ± 2.8	0.1112
4.8	41.4 ± 0.6	26.9 ± 0.6	35.0 ± 0.9	0.1092
5.3	39.5 ± 2.0	26.6 ± 0.3	32.6 ± 2.0	0.0967

^aEnzymatic hydrolysis of Avicel was carried out with cellulose concentration of 10 mg/mL and cellulase concentration of 0.3 mg/mL at 50 ºC. Cellulose conversion was determined at 24 h

^bVanillin concentration was 5 mg/mL