Table 3.

Effect of temperature on vanillin inhibition

Temperature (ºC)	Cellulose conversiona without vanillin (%)	Cellulose conversion with vanillinb (%)	Apparent inhibition (%)	Inhibitor-binding constant β (vanillin, mL/mg)
30	22.0 ± 1.7	14.4 ± 0.2	34.4 ± 3.0	0.1048
40	35.7 ± 0.6	22.9 ± 0.5	35.9 ± 1.6	0.1121
50	41.4 ± 0.6	26.9 ± 0.6	35.0 ± 0.9	0.1092

^aEnzymatic hydrolysis of Avicel was carried out with cellulose concentration of 10 mg/mL and cellulase concentration of 0.3 mg/mL at pH 4.8. Cellulose conversion was determined at 24 h

^bVanillin concentration was 5 mg/mL