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LC-MS/MS analysis of dimethyl-labeled myoglobin; ∼1 pmol, gel-bound sample was analyzed by nano LC-MS/MS. A) Base peak over 50 min acetonitrile gradient. B) Extracted ion chromatogram of underivatized Nt peptide at m/z: 908.455. C) Nt-dimethylated peptide at m/z: 922.471. D) Nt-dimethylated and deamidated peptide at m/z: 922.968. E) Nt-dimethylated peptide with 2 deamidations at m/z: 923.459.
