Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 7;27(2):61–74. doi: 10.7171/jbt.16-2702-002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Association of Biomolecular Resource Facilities

PMC Copyright notice

Figure 9. — MS/MS analysis and identification of the Nt tryptic peptide-dimethyl SMEELEVR in sample 831MT_F (A) and sample 831MT_T (B). C) We show the representation of the experimental data that identified the fragment of FMRP in samples 831MT_T and 831MT_F. All green residues have been confirmed with >95% probability by the Scaffold FDR algorithm. There was only 1 tryptic peptides that identifies the Nt-dimethylation: peptide spanning aa 1–10 of the expressed protein. Expressed fragment of the FMR_1 protein has 33 lysine residues. Experimental data map 26 lysines; 11/26 lysines are found to be dimethylated (see mapped, green regions with “dimethyl lysines”); and nearly all are internal (miscleaved) lysines. D and E) Sequencing result (de novo), confirming Nt-dimethylated peptides SMEELVVEVR for samples 831MT_T and 831MT_F, respectively, that identified FMRP.

Figure 9. — MS/MS analysis and identification of the Nt tryptic peptide-dimethyl SMEELEVR in sample 831MT_F (A) and sample 831MT_T (B). C) We show the representation of the experimental data that identified the fragment of FMRP in samples 831MT_T and 831MT_F. All green residues have been confirmed with >95% probability by the Scaffold FDR algorithm. There was only 1 tryptic peptides that identifies the Nt-dimethylation: peptide spanning aa 1–10 of the expressed protein. Expressed fragment of the FMR_1 protein has 33 lysine residues. Experimental data map 26 lysines; 11/26 lysines are found to be dimethylated (see mapped, green regions with “dimethyl lysines”); and nearly all are internal (miscleaved) lysines. D and E) Sequencing result (de novo), confirming Nt-dimethylated peptides SMEELVVEVR for samples 831MT_T and 831MT_F, respectively, that identified FMRP.