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. 2016 Mar 22;7:46. doi: 10.1186/s13287-016-0305-4

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© Arutyunyan et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10³ cells per well) were cultured in 48-well plates coated with Matrigel. (Right) Representative images of morphological changes of networks. Scale bar 100 μm. (Left) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs (red) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells (green) were only associated with it. Scale bar 100 μm. c Cluster formation. (Left) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. (Right) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. *p <0.05. h hours, UC-MSC umbilical cord-derived mesenchymal stromal/stem cell, VEGF vascular endothelial growth factor (Color figure online)