Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 22;7:46. doi: 10.1186/s13287-016-0305-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Arutyunyan et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 5 — Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31⁺ cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs (red). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 (green). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)