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(A) Matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) MS of Mb-N-Br and unreacted Mb/Mb-N-CHO. (B) Measured isotope distribution with a characteristic isotopic pattern of Br in the b2 fragment ion of Mb-N-Br as determined by collisionally activated dissociation ion-ion proton transfer CAD/IIPT Orbitrap mass spectrometry. The values in parentheses are the difference between the experimentally measured mass and theoretical values.
