Fig. 2.

Colonic CCL25 Expression under Non- and Pro-Inflammatory Conditions. CCL25 protein expression was determined by western blots post-immunoprecipitation, I.P. [A], probing for CCL25 in tissue lysates of resected terminal ileum/small bowel (SB), large bowel afflicted with ulcerative colitis (UC) and non-inflamed colon (NC). Band sizes in keeping with dimeric CCL25 are illustrated for samples probed following I.P. (+), the paired cleared lysate (−), and β-actin (housekeeping; cleared lysates only). Membranes bearing NC samples were subject to greater exposure times given the relatively low abundance/absence of CCL25 under non-inflammatory conditions and are therefore shown as a separate group. Differential expression of CCL25 in tissue samples was confirmed by ELISA [B]. Correlation with inflammatory burden was assessed in mucosal biopsy samples obtained during routine, surveillance colonoscopy (n = 37) by qRT-PCR. Given that CCL25 gene expression was not detectable in non-inflamed colon (control), data are presented relative to GUS-β (housekeeping gene) across endoscopic Mayo severity scores [C] and in correlation with TNFα expression [D]. Asterisks are indicative of statistically significant differences.