Figure 1.

Dietary vitamin A dose dependently altered nuclear localized CTNNB1 and WNT7A in telogen hair follicles of C3H/HeJ mice. Immunohistochemistry was performed with antibodies against CTNNB1 (a–e), WNT7A (f–j), and WNT7B (k–o) in dorsal skin from C3H/HeJ mice fed 0 (a, f, k), 4 (b, g, l), 12 (c, h, m), and 28 (d, i, n) IU vitamin A/g diet. Blinded IHC score for CTNNB1 and WNT7A, WNT7B were calculated as the immunoreactivity (IR) measured on a 0–4 scale multiplied by the fraction of cells within the telogen hair follicle and the fraction of telogen hair follicles. Mean ± SD. Different letters are significantly different, P < 0.05. Bar = 10.1 μM